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洋書 | 技術書

Field Guide to Microscopy
商品コード: 9780819472465

Field Guide to Microscopy

販売価格(税込) 4,830 円
ポイント: 48 Pt
個  数

カゴに入れる

Tomasz S. Tkaczyk
156pages Spiral Bound
2010/5/18
FG13

詳細

This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics. This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and array microscopy, CARS, and SPIM.

Glossary of Symbols

Basic Concepts
  The Nature of Light
  Spectrum of Light vs. Microscopy
  Wave Equations
  Wavefront Propagation
  Optical Path Length (OPL)
  Reflection and Refraction Laws
  Total Internal Reflection
  Evanescent Wave in TIR
  Propagation through Anisotropic Media
  Polarization of Light and Polarization States
  Coherence and Monochromatic Light
  Interference
  Contrast versus Spatial and Temporal Coherence
  Contrast of Fringes (Polarization and Amplitude Ratio)
  Multiple Wave Interference
  Interferometers
  Diffraction
  Diffraction Grating
  Useful Definitions of Geometrical Optics
  Image Formation
  Magnifications
  Stops and Rays in Optical System
  Aberrations
  Chromatic Aberrations
  Spherical and Coma Aberrations
  Astigmatism, Field Curvature and Distortion
  Performance Metrics

Microscope Construction
  Compound Microscope
  The Eye
  Upright and Inverted Microscopes
  Finite Tube Length Microscope
  Infinity Corrected Systems
  Telecentricity of a Microscope
  Magnification of the Microscope
  Numerical Aperture
  Rayleigh versus Abbe Resolution Limit
  Useful Magnification
  Depth of Field and Depth of Focus
  Magnification and Frequency versus DOF
  Koehler Illumination
  Alignment of Koehler Illumination
  Critical Illumination
  Stereo Microscopes
  Eyepieces
  Nomenclature and Marking of Objectives
  Objective Designs
  Special Objectives and Features
  Special Lens Components
  Cover Glass and Immersion
  Common Light Sources for Microscopy
  LED Light Sources
  Filters
  Polarizers and Polarization Prisms

Specialized Techniques
  Microscope Objects
  Selection of Microscopy Technique
  Image Comparison
  Phase Contrast
  Visibility in Phase Contrast
  The Phase Contrast Microscope
  Characteristic Features of Phase Contrast Microscopy
  Amplitude Contrast
  Oblique Illumination
  Modulation Contrast - Hoffman Contrast
  Dark Field Microscopy
  Optical Staining: Rheinberg Illumination
  Optical Staining: Dispersion Staining
  Shearing Interferometry - Principle of DIC
  DIC Microscope Design
  Appearance of DIC Images
  Reflectance DIC
  Polarization Microscopy
  Images obtained with Polarization Microscopes
  Compensators
  Confocal Microscopy
  Scanning Approaches
  Images from Confocal Microscope
  Fluorescence
  Configuration of Fluorescence Microscope
  Images from Fluorescence Microscopy
  Properties of Fluorophores
  Single versus Multi-Photon Excitation
  Light Sources for Scanning Microscopy
  Practical Considerations on LSM
  Short Coherence Microscopy
  Optical Profiling Techniques
  Optical Profilometry - System Design
  PSF Algorithms

Resolution Enhancement Techniques
  Structured Illumination - Axial Sectioning
  Structured Illumination - Resolution Enhancement
  TIRF Microscopy
  Solid Immersion
  Stimulated Emission Depletion
  STORM
  4Pi Microscopy
  Limits of Light Microscopy

Other Techniques
  Raman and CARS Microscopy
  SPIM
  Array Microscopy

Digital Microscopy and CCD
  Digital Microscopy
  CCD Principle
  CCD Architectures
  Noise in CCD
  SNR and Digitization of CCD
  CCD Sampling

Equation Summary
Bibliography
Index

Sample Pages

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